Purification and Regulation of Glucose-6-Phosphate Dehydrogenase from Obligate Methanol-Utilizing Bacterium Methylomonas M15
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چکیده
منابع مشابه
Purification and properties of Penicillium glucose 6-phosphate dehydrogenase.
1. Glucose 6-phosphate dehydrogenase was isolated and partially purified from a thermophilic fungus, Penicillium duponti, and a mesophilic fungus, Penicillium notatum. 2. The molecular weight of the P. duponti enzyme was found to be 120000+/-10000 by gelfiltration and sucrose-density-gradient-centrifugation techniques. No NADP(+)- or glucose 6-phosphate-induced change in molecular weight could ...
متن کاملPurification and properties of d-glucose-6-phosphate dehydrogenase.
n-Glucose-6-phosphate dehydrogenase (Zwischenferment) was discovered by Warburg and Christian in 1931 (1, 2). An active preparation was obtained then from horse erythrocytes and in 1932 from the Lebedev juice made from brewers’ yeast (3). Because of its extreme specificity, this enzyme has become an important analytical tool. Various preparations of it have been described, the first being those...
متن کاملGlucose-6-Phosphate Dehydrogenase Deficiency and Neonatal Hyperbilirubinemia
Background: Jaundice is affecting over 60-80 percent of neonates in the first week of life. Glucose-6-phosphate dehydrogenase (G6PD) deficiency, which is an important cause of pathologic hyperbilirubinemia, can lead to hemolytic anemia, jaundice and kernicterus. The present study was performed to determine the prevalence of G6PD deficiency among icteric neonates in Shirvan, Iran. Methods: This...
متن کاملGlucose-6-phosphate Dehydrogenase PURIFICATION AND PARTIAL CHARACTERIZATION
Glucose-6-phosphate dehydrogenase has been purified lOOO-fold from pig liver. This enzyme exists as an active dimer of molecular weight 133,000 and an inactive monomer of molecular weight 67,500. The pH of maximum activity is 8.5 and the ionic strength maximum is 0.1 to 0.5 M. Glucose-6-phosphate dehydrogenase is highly specific for NADP+ and glucose B-phosphate. Apparent K, values of 3.6 pM an...
متن کاملGlucose-6-Phosphate Dehydrogenase
Description Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most common human enzyme deficiency that mainly affects the red blood cells (RBCs). A defect in G6PD enzyme leads to the destruction of premature RBCs causing hemolytic anemia because the body can not compensate the destroyed RBCs. Thus, the affected individuals show jaundice (paleness, yellowing of the skin and whites of th...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1978
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1978.tb12390.x